BP500-40 대역 통과 필터(CWL=500nm, FWHM=40nm)

Fluorescence Microscopy (Biomedical)

This is the most likely high-value application for an OD5 filter of this type.

• YFP Excitation: The filter covers the 480–520 nm range, which is the perfect excitation band for Yellow Fluorescent Protein (YFP) (Excitation max ~514 nm) and related fluorophores like SYBR Green (Excitation ~497 nm).
• CFP Emission: It can serve as an emission filter for Cyan Fluorescent Protein (CFP) (Emission max ~475–505 nm), effectively capturing the fluorescence while blocking the excitation source (if the excitation is <460 nm).
• FITC/GFP (Widefield): While standard GFP filters are often narrower (e.g., 470/40), this filter captures the peak emission of GFP (509 nm) and FITC (519 nm).

Recommended Light Source

• YFP Excitation

Cyan/Green LEDs or Argon Lasers: Since the filter passes 480–520 nm, you need a source rich in this "cyan" region to efficiently excite Yellow Fluorescent Protein (YFP).

• CFP Emission

Deep Blue or Violet LEDs (<460 nm): Here, the filter collects the signal (480–520 nm). You need a light source that is spectrally distant enough to be blocked by the filter's OD5 blocking range (specifically below 460 nm).

• FITC/GFP (Widefield)

Standard Blue LEDs (470 nm): While this filter is wider than standard GFP emission filters, it captures the peak emission of GFP (509 nm).

Astronomy (Visual & EAA)

• OIII & H-Beta: The 480–520 nm range perfectly centers on the H-Beta line (486.1 nm) and the OIII lines (495.9 nm & 500.7 nm).
• Use Case: This acts as a "Broadband Nebula Filter" (UHC type), isolating nebulae from light pollution (sodium/mercury streetlights) with high efficiency.

Recommended Light Source

• Emission Nebulae (H-Beta & OIII)

This filter acts as a UHC (Ultra-High Contrast) filter. It is designed to capture the specific glow of ionized gas clouds while blocking the rest of the spectrum.

1. Doubly Ionized Oxygen (OIII)
2. Hydrogen-Beta (H-β)

"Broadband" Nebula Sources

Because the BP500-40 has a 40nm bandwidth (wide for a nebula filter), it captures both OIII and H-Beta simultaneously. This makes it ideal for complex nebulae that emit light in multiple chemicals.

1. Orion Nebula (M42)
2. Lagoon Nebula (M8)