BP525-20 대역 통과 필터(CWL=525nm, FWHM=20nm)

Fluorescence Microscopy (Emission Filter)

This is the most common use. 525 nm is the peak emission wavelength for many popular green fluorescent dyes and proteins. The OD6 blocking is critical here to block the bright excitation light so the camera detects only the faint fluorescence.

• Target Fluorophores: GFP (Green Fluorescent Protein), FITC (Fluorescein), Alexa Fluor 488, Cy2, and SYBR Green.

Recommended Light Sources

To use this filter effectively, your light source must emit energy within the 515–535 nm range.

• High-Power Green LEDs (Recommended): Look for LEDs with a peak wavelength of 520 nm, 525 nm, or 530 nm. These are the ideal match for this filter.
• Laser Diodes (520 nm): Direct green laser diodes (often 520 nm) fit perfectly within the passband.
• DPSS Lasers (532 nm): A 532 nm green laser is common, but it sits right at the edge of this filter's 20 nm bandwidth (515–535 nm). It will pass, but you might lose some intensity if the filter's angle isn't perfect. A slightly wider filter (e.g., BP525-25 or BP532) is usually preferred for 532 nm lasers, but BP525-20 can work.
• Broadband Sources (Xenon/Mercury Arc): Only if used with a corresponding excitation filter to stimulate fluorescence.

Machine Vision & Imaging:

Used to increase contrast in industrial inspection. A narrow 20 nm bandwidth effectively blocks ambient sunlight and overhead lighting, allowing the camera to see only the light from a structured green LED source.

Laser Clean-up

It can be used to "clean up" the output of a laser, ensuring only the 525 nm line passes through, removing spectral noise.

The specific required light sources are:

• 520 nm Direct Diode Laser: This is the ideal source. It sits comfortably in the middle of the filter's range, ensuring maximum transmission while the filter strips away any "wings" or spontaneous emission noise.
• 532 nm DPSS Laser: This is the most common green laser. It fits within the filter's passband (which ends at roughly 535 nm), effectively removing the infrared pump light (typically 808 nm or 1064 nm) that often leaks through these lasers.