SP750 쇼트패스 필터(T>95%@420-740nm, OD6@760-1200nm)

Multiphoton (Two-Photon) Microscopy

This specification is a classic match for an Emission Filter in Multiphoton Microscopy.

• How it works: In multiphoton microscopy, you use a high-power infrared laser to penetrate deep into biological tissue. This laser excites fluorescent dyes in the sample, which then emit visible light.
• The Filter's Job: You need to detect the faint visible glow (fluorescence) while blocking the blindingly bright infrared laser that acts as the excitation source. Because the excitation laser is so powerful, you need an OD6 rating (1,000,000:1 rejection ratio) to ensure none of that laser light reaches your sensitive detector (PMT or Camera).

Request Light Source:

• For Multiphoton (Two-Photon) Microscopy, the light source is the most critical component of the entire system. Because this technique relies on "non-linear" optics (two photons hitting a molecule at the exact same femtosecond), you cannot use standard continuous lamps or diode lasers. You must use Ultrafast Pulsed Lasers.

Raman Spectroscopy (Anti-Stokes)

It can be used to block a 785nm or 830nm Raman laser line while analyzing "Anti-Stokes" scattering (signals shifted to shorter wavelengths/higher energy than the laser).

Recommended Light Sources

The "light source" you use depends on which side of the filter you are on.

A. Sources the filter is designed to BLOCK (Excitation Sources)

These are the sources you would use to illuminate or excite your sample. The filter prevents this light from blinding the detector.

• Ti:Sapphire Lasers (Tunable): The most common source for multiphoton microscopy, usually tuned between 760nm and 1000nm.
• Nd:YAG Lasers: Operating at 1064nm. This filter blocks this wavelength completely.
• NIR Diode Lasers: Common wavelengths like 785nm, 808nm, 830nm, or 980nm.

B. Sources the filter is designed to TRANSMIT (Signal Sources)

These are the sources (or signals) that will pass through the filter to be seen by your eye or camera.

• Fluorophores (Fluorescent Dyes):DAPI / Hoechst (Blue emission ~460nm)GFP / FITC (Green emission ~510nm)RFP / mCherry / Texas Red (Red emission ~610nm)Note: It will transmit Cy5 (emission ~670nm) but will cut off dyes that emit deeper into the NIR (like Cy7).
• Broadband White Light (Halogen/Xenon): If used in a projection or illumination system, this filter effectively "cleans" the beam, removing the heat (IR) and transmitting "cool" visible light.