MultiBandpass Filter 450/520/596

Triple-Band Fluorescence Filter for Multi-Color Imaging

The MultiBandpass Filter 450/520/596 delivers simultaneous transmission across three precisely defined spectral windows—ideal for multi-fluorophore microscopy, flow cytometry, and advanced imaging workflows. Engineered with hard-coated dielectric layers, this filter provides sharp band edges, high transmission efficiency, and deep blocking between passbands to eliminate crosstalk.

Key Features

• Three discrete passbands: 450 nm (blue), 520 nm (green), and 596 nm (orange/red) for common fluorophore combinations
• High transmission: Optimized coating design ensures >90% peak transmission in each passband
• Deep inter-band blocking: OD4+ rejection between bands prevents spectral bleed-through
• Durable hard coating: Resistant to humidity, temperature cycling, and cleaning protocols
• Precision substrate: Low autofluorescence, λ/4 surface quality for diffraction-limited performance

Applications

• Multi-color fluorescence microscopy (DAPI/GFP/mCherry, Hoechst/FITC/Texas Red)
• Flow cytometry and cell sorting with multiple markers
• High-content screening and automated imaging platforms
• Spectral unmixing and ratiometric imaging experiments

Technical Advantages

Combining three bandpass regions in a single optic simplifies optical train design, reduces alignment complexity, and minimizes light loss compared to sequential filter wheels. The rugged coating withstands repeated cleaning and maintains spectral performance over thousands of hours of use in demanding lab environments.