BP485-20 대역 통과 필터(CWL=485nm, FWHM=20nm)

Fluorescence Microscopy and Lab Assays

Scientists use special glowing dyes (like FITC or Green Fluorescent Protein) to light up microscopic structures inside cells. These dyes need a specific blue-cyan light (around 485nm) to "wake up" and glow. Because this filter blocks 99.9999% of unwanted light from UV to infrared (that is what the OD6 means) but lets 95% of the right blue light through, it provides a incredibly pure beam. This prevents background glare and creates incredibly crisp, high-contrast images of the glowing samples without washing them out.

Recommended light source:

• Broadband Arc Lamp (Xenon or Mercury) light source: These produce a very bright, intense white light containing all colors. The advantage is raw power, and this heavy-duty filter easily handles slicing out exactly the 485nm blue portion while completely blocking the overwhelming white glare.
• Blue LED (470nm-490nm) light source: LEDs are cost-effective, run cool, and have a long lifespan. The advantage here is that the filter acts as a precise "clean-up" tool, trimming the slightly messy, fuzzy edges of the LED's color spectrum to make the light perfectly sharp and pure for sensitive lab tests.

Flow Cytometry (Automated Cell Sorting)

This is a medical and research technique where machines rapidly count, analyze, and sort thousands of individual cells per second as they flow in a tiny liquid stream. The cells are coated with blue-light-sensitive glowing tags. The filter is used within the machine's optics to perfectly isolate the exact blue light needed. The incredibly high 95% transmission is critical here because it ensures the machine's sensors don't miss even the faintest, fastest light signals coming from single cells.

Recommended light source:

• 488nm Laser (Solid-State or Argon) light source: This is the absolute industry standard for cell sorting machines because it provides an extremely focused, high-energy beam. The advantage is that the filter acts as a "laser line clean-up" tool, removing any tiny bits of unwanted background light (optical noise) leaking from the laser equipment, ensuring the machine counts with perfect accuracy.
• Supercontinuum (White Light) Laser light source: These highly advanced lasers shoot a powerful, tightly focused beam containing every color at once, offering incredible flexibility for complex research. The advantage is that researchers can use this filter to cleanly extract just the 485nm blue band they need from the rainbow beam for a highly specific cellular test.