Dye for Green Channel
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| Fluorophore Family | Manufacturer | λEx/λEm Peak (nm) | Key Performance Characteristic | Cost/General Use |
|---|---|---|---|---|
| Alexa Fluor 488 | Invitrogen/Thermo Fisher | 495/519 | Gold Standard. High photostability, bright, pH-insensitive. | High |
| FITC (Fluorescein) | Generic (Multiple) | 495/519 | Classic Standard. Spectrally identical. Major drawback: Poor photostability, pH-sensitive (dimmer in acidic environments). | Low |
| EGFP / GFP | N/A (Fluorescent Protein) | 488/507 | Live-Cell Imaging. Genetically encoded, allowing for internal expression. | N/A (Cloning) |
| DyLight 488 | Thermo Fisher / Abcam | 493/518 | Excellent Balance. High photostability and brightness, often used as a cost-effective alternative to Alexa Fluor 488. | Mid-Range |
| CF488A | Biotium | 490/515 | Bright & Stable. Designed for high performance, a direct competitor to Alexa Fluor 488. | Mid-Range |
| iFluor 488 | AAT Bioquest | 493/516 | Bright & Hydrophilic. Designed for high brightness and better conjugation properties. | Mid-Range |
| Oregon Green 488 | Invitrogen/Thermo Fisher | 492/517 | Highly Photostable. More photostable than FITC, but often less bright than Alexa Fluor 488. | Mid-Range |
| Cy2 | Cytiva | 492/510 | Legacy Dye. Older cyanine dye, spectrally similar but generally much less stable than the newer alternatives. | Low |
