What is Stokes Shift?
The Stokes shift is simply defined as the difference in wavelength between two critical points for a fluorochrome:
1. The peak excitation intensity (the maximum wavelength of light absorbed).
2. The peak emission intensity (the maximum wavelength of light radiated as fluorescence).
Since fluorescence involves absorbing light and then radiating light of a lower energy, the emitted light always has a longer wavelength than the excitation light.
