525nm Bandpass Filter

525nm bandpass filters are designed to transmit a selected green wavelength band centered near 525nm while reducing unwanted out-of-band light.

Applications:

  • Green fluorescence emission collection (such as GFP, FITC, and Alexa Fluor 488)
  • Green LED source cleanup for machine vision, inspection, and illumination systems
  • Detector-side green signal isolation before cameras, photodiodes, PMTs, or spectrometers
  • Biomedical sensing, flow cytometry, and optical measurement setups where the target signal is near 525nm

Bandwidth options:

  • 10nm FWHM for tight 525nm signal isolation and maximum background noise rejection
  • 20nm FWHM for balanced signal transmission and out-of-band background reduction
  • 40nm and 50nm FWHM for broader green emission collection, higher signal levels, or accommodating angular shifts

Select the bandwidth based on the source spectrum, emission signal, detector sensitivity, blocking range, and required signal level.
525nm Bandpass Filter
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Filters

6 items
Center Wavelength (nm)
FWHM (nm)
Optical Density(OD)

Filter

Center Wavelength (nm)
FWHM (nm)
Optical Density(OD)
US20020045260A1 - Method of isolating stem cells

US20020045260A1 - Method of isolating stem cells

Context: This patent describes methods for isolating specific stem cell populations (such as hematopoietic stem cells) from bone marrow or blood using flow cytometry and fluorescent labeling of cell surface markers.

Usage of Filter: The filter is placed in front of the "FL1" photodetector within the flow cytometer's optical bench.

Function: It serves as a spectral selection window that specifically transmits light in the 515–535nm range while blocking excitation laser light (488nm) and autofluorescence from other channels.

Result: It isolates the specific green emission signal from FITC-conjugated antibodies (e.g., Anti-Sca-1 or Anti-c-kit) bound to the stem cells, allowing the machine to accurately sort and collect the target cell population.

US8298792B2 - Four-color DNA sequencing by synthesis

US8298792B2 - Four-color DNA sequencing by synthesis

Context: This patent covers "Sequencing-by-Synthesis" technology (foundational to Illumina sequencers), where DNA is sequenced by detecting four different fluorescently labeled nucleotides incorporated into a growing DNA strand.

Usage of Filter: The filter is used as one of four emission filters in the fluorescence imaging system, specifically assigned to the "Green" detection channel.

Function: It discriminates the emission of the shortest-wavelength fluorophore (often attached to the Thymine or Cytosine base in this specific chemistry) from the other three longer-wavelength dyes (yellow, red, far-red) used in the mixture.

Result: It ensures high signal-to-noise ratio for the green channel, enabling the sequencer to correctly identify the base at that position in the DNA sequence without "crosstalk" error from the other dyes.

US6198107B1 - Fluorometric detection using visible light

US6198107B1 - Fluorometric detection using visible light

Context: This patent describes a "Dark Reader" type device for visualizing DNA in electrophoresis gels using visible blue light excitation instead of UV light, which prevents damage to the DNA samples.

Usage of Filter: The filter is positioned as a viewing screen or camera lens filter between the illuminated sample and the observer/detector.

Function: It acts as a blocking filter that rejects the intense blue excitation light (approx. 460–480nm) while maximizing transmission at the peak emission of DNA stains like SYBR Green I or GFP (which peak near 520-525nm).

Result: The user sees the DNA bands glowing brightly (green) against a dark background, providing high-contrast visualization without the safety hazards of UV radiation.

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