575nm Bandpass Filter

575nm light, a yellow-green wavelength in the visible spectrum, offers high penetrability and moderate energy, making it suitable for applications requiring precise spectral isolation.

  • Application 1: In fluorescence microscopy, the 575nm Bandpass Filter isolates emission signals from yellow-green fluorescent dyes (e.g., Alexa Fluor 575), enhancing image contrast by blocking unwanted wavelengths.
  • Application 2: For industrial color sorting systems, it enables accurate detection of materials with specific yellow-green spectral reflectance, such as sorting fruits or plastics by their surface pigmentation
  • Application 3: In plant physiology research, the filter helps analyze chlorophyll fluorescence at 575nm, assessing photosynthetic activity by filtering out ambient light and focusing on the target spectral band.
575nm Bandpass Filter
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US6683314B2 - Fluorescence detection instrument with reflective transfer leg beam splitters

US6683314B2 - Fluorescence detection instrument with reflective transfer leg beam splitters

Context: This patent describes a sophisticated optical system for a multi-color flow cytometer. The system is designed to excite biological particles (like cells) with lasers and then collect/separate the resulting fluorescent emissions into distinct color bands for analysis.

Usage of Filter: The 575nm bandpass filter (typically specified as ~575/26nm) is used in the detector array path specifically to target the emission of the fluorophore Phycoerythrin (PE).

Function: Its function is to selectively transmit the yellow-orange light (centered at 575nm) emitted by PE-labeled antibodies while vigorously blocking:

  • The excitation laser light (usually 488nm or 532nm).
  • Fluorescence from other nearby fluorophores (such as Fluorescein/FITC which peaks at ~520nm).

Result: The filter achieves high-purity signal isolation, allowing the instrument to accurately quantify the presence of specific surface markers on a cell without "crosstalk" from other dyes, enabling precise multi-parametric cellular analysis.

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