T＞90%@565-800nm+R＞99%@510-540nm,Longpass Dichroic Mirror(LPDM552)

Fluorescence Microscopy

The most likely application for this mirror is inside a fluorescence microscope filter cube designed for Green Excitation / Red Emission.

How it works:

1. Reflects Excitation: It reflects the green excitation light (510–540 nm) down onto the sample. The high reflectivity ($>99\%$) ensures maximum laser/light power reaches the specimen.
2. Transmits Emission: The sample fluoresces (emits light) at a longer wavelength (Red). This signal ($>565$ nm) passes through the mirror to the camera or eyepiece.

Target Fluorophores:

This mirror is perfectly optimized for dyes and proteins that are excited by green light and emit red light.

• TRITC (Tetramethylrhodamine)
• Cy3 (Cyanine 3)
• RFP (Red Fluorescent Protein) / DsRed
• Alexa Fluor 532 / 546
• Rhodamine B

Recommended Light Sources

A. Lasers (Ideal for the R>99% spec)

The high reflectivity suggests this mirror is robust enough for laser applications to minimize power loss.

• 532 nm DPSS Laser: The most common green laser. This fits perfectly in the center of the reflection band.
• 514 nm Argon Laser: A classic gas laser line that also fits well.
• 543 nm HeNe Laser: Green Helium-Neon laser (matches the upper edge of the reflection band).

B. Incoherent Sources (Microscopy)

• Green LED: High-power LEDs centered around 520 nm or 530 nm.
• Arc Lamps (Mercury/Xenon): Used with an Excitation Filter (Bandpass 510–540 nm) placed before the dichroic mirror to block other wavelengths.

Laser Beam Combining

This mirror can also be used on an optical bench to combine or separate laser beams of different colors.

• Combiner: You can combine a Green Laser (e.g., 532 nm) and a Red Laser (e.g., 633 nm) into a single coaxial path. The green laser reflects off the mirror, while the red laser passes through it from behind.

Recommended Light Sources

A. The Reflected Source (Green) - 532 nm DPSS Laser

• Why: This is the most common green laser wavelength. It sits perfectly in the center of the mirror's high-reflectivity band (510–540 nm).
• Performance: The mirror will reflect $>99\%$ of this beam at a 45° angle, redirecting it into the combined path with almost no power loss.
• Alternatives: 514 nm (Argon) or 520 nm (Diode).

B. The Transmitted Source (Red) - 632.8 nm HeNe Laser or 635–660 nm Diode Laser

• Why: These red wavelengths are well above the mirror's 565 nm cut-on point.
• Performance: The mirror is transparent to these wavelengths ($T > 90\%$). You place this laser behind the mirror so it shines through the glass and merges with the reflected green beam on the other side.