525nm 대역 통과 필터

Context: This patent describes methods for isolating specific stem cell populations (such as hematopoietic stem cells) from bone marrow or blood using flow cytometry and fluorescent labeling of cell surface markers.

Usage of Filter: The filter is placed in front of the "FL1" photodetector within the flow cytometer's optical bench.

Function: It serves as a spectral selection window that specifically transmits light in the 515–535nm range while blocking excitation laser light (488nm) and autofluorescence from other channels.

Result: It isolates the specific green emission signal from FITC-conjugated antibodies (e.g., Anti-Sca-1 or Anti-c-kit) bound to the stem cells, allowing the machine to accurately sort and collect the target cell population.

Context: This patent covers "Sequencing-by-Synthesis" technology (foundational to Illumina sequencers), where DNA is sequenced by detecting four different fluorescently labeled nucleotides incorporated into a growing DNA strand.

Usage of Filter: The filter is used as one of four emission filters in the fluorescence imaging system, specifically assigned to the "Green" detection channel.

Function: It discriminates the emission of the shortest-wavelength fluorophore (often attached to the Thymine or Cytosine base in this specific chemistry) from the other three longer-wavelength dyes (yellow, red, far-red) used in the mixture.

Result: It ensures high signal-to-noise ratio for the green channel, enabling the sequencer to correctly identify the base at that position in the DNA sequence without "crosstalk" error from the other dyes.

Context: This patent describes a "Dark Reader" type device for visualizing DNA in electrophoresis gels using visible blue light excitation instead of UV light, which prevents damage to the DNA samples.

Usage of Filter: The filter is positioned as a viewing screen or camera lens filter between the illuminated sample and the observer/detector.

Function: It acts as a blocking filter that rejects the intense blue excitation light (approx. 460–480nm) while maximizing transmission at the peak emission of DNA stains like SYBR Green I or GFP (which peak near 520-525nm).

Result: The user sees the DNA bands glowing brightly (green) against a dark background, providing high-contrast visualization without the safety hazards of UV radiation.