ORANGE Channel Filter Set - Ex:BP545/25nm,Em:BP605/55nm,Dm:LPDM555

Fluorophores

• TRITC (Tetramethylrhodamine): The classic target. It emits a bright orange-red glow.
• Cy3 (Cyanine 3): Very common alternative to TRITC; this filter set is highly efficient for Cy3.
• Alexa Fluor 546 / 555: Brighter, more stable modern alternatives to TRITC.
• DsRed / RFP (Red Fluorescent Protein): Often usable, though the excitation peak of RFP (558 nm) is slightly on the edge of your excitation filter (545/25).
• Propidium Iodide (PI): A DNA stain used to identify dead cells.

Recommended Light Sources

• Mercury Arc Lamp (HBO): These lamps have a massive spectral peak exactly at 546 nm, which matches the center of your excitation filter perfectly.
• Green LED (High Power): Look for LEDs centered at 540 nm, 545 nm, or 550 nm. These are efficient, cool, and stable. (Avoid "Lime" LEDs if they center closer to 565 nm).
• Metal Halide Lamp: Provides strong green output, though generally less intense at the specific 546 nm peak than Mercury.
• Lasers (532 nm): A solid-state green laser at 532 nm fits just inside the excitation window.

FISH (Fluorescence In Situ Hybridization)

Detecting specific gene sequences on chromosomes using orange probes.

Recommended Light Sources

• Mercury Arc Lamp (HBO): Mercury lamps have a massive, natural spectral peak at exactly 546 nm. Your excitation filter (BP545/25) is engineered specifically to capture this intense peak. This results in the highest possible excitation energy for orange probes, making faint gene signals pop against the background.
• High-Power Green/Lime LED: Modern LEDs are replacing mercury lamps for FISH due to their stability, which is crucial when trying to capture images of tiny, faint chromosomal dots without them flickering or fading.
• Laser Sources - 532 nm Solid State Laser: This fits just inside the lower edge of your filter window and works well for TRITC/Cy3.