Fluorescence
Comparison of different Light Sources in Fluoro...
Optical filters must be precisely designed to match the specific spectral output of the light source used in fluorescence microscopy. Filter requirements vary dramatically depending on the illumination source. Light...
Comparison of different Light Sources in Fluoro...
Optical filters must be precisely designed to match the specific spectral output of the light source used in fluorescence microscopy. Filter requirements vary dramatically depending on the illumination source. Light...
What is Stokes Shift?
The Stokes shift is simply defined as the difference in wavelength between two critical points for a fluorochrome: 1. The peak excitation intensity (the maximum wavelength of light absorbed). 2. The...
What is Stokes Shift?
The Stokes shift is simply defined as the difference in wavelength between two critical points for a fluorochrome: 1. The peak excitation intensity (the maximum wavelength of light absorbed). 2. The...
Dye for Green Channel
Fluorophore Family Manufacturer λEx/λEm Peak (nm) Key Performance Characteristic Cost/General Use Alexa Fluor 488 Invitrogen/Thermo Fisher 495/519 Gold Standard. High photostability, bright, pH-insensitive. High FITC (Fluorescein) Generic (Multiple) 495/519 Classic...
Dye for Green Channel
Fluorophore Family Manufacturer λEx/λEm Peak (nm) Key Performance Characteristic Cost/General Use Alexa Fluor 488 Invitrogen/Thermo Fisher 495/519 Gold Standard. High photostability, bright, pH-insensitive. High FITC (Fluorescein) Generic (Multiple) 495/519 Classic...